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The plain-English version
Most users run 100 to 300 micrograms (a tiny subQ shot) before bed, for 5 to 10 days. Set expectations low - the 1970s claim that named this peptide has not held up in modern follow-up research. Always with a doctor.
Route: subcutaneous injection. Evidence tier for the dose: Weak anecdotal. Anecdotal reports exist but without strong mechanistic backing for this specific use.
The dose at a glance
| What's typical | What's the max in research |
|---|---|
| Starting dose: 100 mcg subQ (under-the-skin shot) 30 minutes before bed for the first 3 nights. | Max observed in research: Early Russian protocols went as high as 1,000 mcg - more was not better and side effects increased. 300 mcg is the practical community ceiling. |
| Maintenance dose: 100 to 300 mcg before bed. | |
| Frequency: Once daily, before main sleep block. Most protocols do not run continuously. | Half-life: Short - estimated 7-15 minutes in plasma. The fast clearance is part of why the original sleep-induction claim is hard to verify. |
Titration (how to ramp the dose)
Most users skip formal titration (slow ramp-up) since starting doses are already tiny.
Cycle length and time off
On cycle: 5 to 10 nights typical. Long-term continuous use is rare and unsupported by data.
Off cycle: At least 1-2 weeks off after a 10-night cycle.
Timing notes: Inject 30 minutes before bed. Inject in stomach or thigh fat. Effect (if any) is most reported in the first 2-3 nights of a cycle.
What's commonly prescribed (per published protocols and clinical write-ups)
US compounding pharmacies will fill DSIP as a lyophilized (freeze-dried, shelf-stable) vial, typically 5 mg or 10 mg. Not FDA-approved for any indication. Most sleep-medicine doctors will not prescribe it; longevity clinics sometimes do.
Source: prescribing labels, published protocols, and clinical write-ups. We have not independently tested each prescriber pattern.
Reconstitution (mixing the vial)
Lyophilized 5 mg vials reconstitute (mix back into liquid) with exactly 2 mL of bacteriostatic water (sterile water with preservative, the kind compounding pharmacies sell for reconstituting peptides). At 5 mg per 2 mL, every 0.04 mL on the syringe equals 100 mcg. If your pharmacy filled the vial with a different volume, recalculate from the mg/mL printed on your vial label - not from this page. Store reconstituted vial in the fridge, use within 30 days.
Reconstitution = mixing freeze-dried (lyophilized) powder with bacteriostatic water (sterile water with preservative) so you can draw a dose into a syringe.
Where to get it
Honest take on where to source this peptide. What fits which user, and what to watch for.
Compounded peptide vendor
Limitless Biotech
Fits: You want a short cycle of DSIP for sleep architecture, you have a prescriber, and you want a US-based source.
Watch: Human evidence for DSIP is genuinely thin. Use as a tool to test, not a daily.
(pending) Visit Limitless Biotech ->We're evaluating vendor partnerships across this category. When we have an active affiliate relationship, we disclose it here clearly. As of now: placeholder or pending status per vendor above. Affiliate links don't change our editorial take. We only recommend what we'd actually use.
Drug interactions to watch
- No documented drug interactions in the published research.
- Theoretical additive effect with melatonin and prescription sleep meds - stack carefully.
Who should not take this (contraindications)
- Pregnancy and breastfeeding (no safety data).
- Known hypersensitivity to the peptide.
- Severe sleep apnea - any sedation-direction agent should be discussed with a sleep-medicine specialist first.
Common side effects
- Vivid dreams or unusual sleep content.
- Mild morning grogginess.
- Injection-site soreness.
Serious side effects (call a doctor)
- Allergic reaction (hives, swelling) - rare.
- Excessive sedation if stacked with other sleep meds.
What to monitor while you're on it
- Sleep diary (bedtime, wake time, subjective quality 1-10) for the entire cycle.
- Wearable data (Oura, Whoop, Apple Watch) if available - the deep-sleep claim is testable.
- Stop and reassess at 5 nights if no perceived effect.
How to stop
Just stop. No taper needed.
Where this dosing comes from
- Schoenenberger and Monnier, original DSIP isolation (1977)
- Schneider-Helmert, follow-up sleep work (1986)
- US compounding pharmacy formularies (Tailor Made, Strive)
- Biohacker community consensus, not clinical data, for current dosing
Where these doses come from
These doses are reported by users and clinics, but the evidence base is genuinely thin. We are tracking, not endorsing.
Always work with a real prescriber. Don't self-prescribe.
Safety reminder: None of this replaces a doctor. Peptides are gray-market in the US for most uses. Talk to a real prescriber before you change anything.
Where to go next
- Full DSIP directory entry - status, sourcing, studies, what to skip
- DSIP for deep sleep induction
- DSIP for stress-related sleep issues
- DSIP for shift-work sleep disruption
- What are peptides - if you skipped the foundation
- How peptides actually work - mechanism in plain English
- Subscribe to the dispatch
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Last reviewed · 2026-05-07 · Page generated by Protocol One matrix engine. None of this replaces a doctor. Peptides are gray-market in the US for most uses. Talk to a real prescriber before you change anything.